Tylosin affects most Gram-positive and some gram-negative bacteria including Streptococcus, Leptospira, Corynebacterium, Clostridium, erysipelothrix, Pasteurella, chlamydia, treponema hyodisenteriae, spirochetes and mycoplasma.
During intramuscular injection antibiotic is quickly distributed throughout the body and reaches maximum concentration in tissue approximately after 1 hour after administration. Therapeutic effect of this antibiotic in tissues retains for at least 20 hours. It is eliminated primarily with urine and with milk in lactating animals.